Optimise Your NGS Experiment Design with NextGenSeek’s Expert Consultancy Services

Are you aware that many NGS experiments fail to meet expectations due to poor library design and experimental setup choices? At NextGenSeek, we provide bioinformatics analysis and pre-experiment advisory services to ensure your research is designed for success. We help researchers avoid common pitfalls and produce reliable, high-quality results by addressing critical considerations in NGS experimental design.

Key Questions We Address in NGS Experimental Design

Our consultancy services focus on ensuring that every aspect of your NGS experiment is optimised, including:

Optimal Coverage Depth

Determining the proper coverage depth is crucial for generating high-quality sequencing data. We help you calculate the appropriate depth based on your research goals, sample type, and sequencing platform, ensuring you capture all relevant genetic variations without wasting resources.

Ideal Read Length for Your Experiment

Choosing the correct read length can significantly impact the accuracy of your results. We guide you in selecting the best read length based on the complexity of your genomic target and the type of analysis you plan to perform, whether it’s whole-genome sequencing, RNA-Seq, or metagenomics.

Paired-End Reads vs. Single-End Sequencing

We help you determine whether paired-end or single-end sequencing suits your research. While paired-end reads provide more comprehensive information, single-end reads can be cost-effective for specific projects. Our consultancy ensures you make the right choice, balancing your need for data depth with cost efficiency.

(Check out our two educational videos on paired-end versus single-end sequencing to help with your decision-making.)

Selecting the Most Suitable Sequencing Technology

With numerous sequencing technologies available, choosing the right one for your project can be challenging. Our experts help you select the best sequencing platform based on factors such as:

  • The type of genomic data you are targeting.

  • Your budget.

  • The desired resolution and throughput of your study.

We ensure you achieve the best possible results by matching your needs to the optimal sequencing technology.

Choosing the Right Library Preparation Kit

Library preparation is a critical step in NGS, and choosing the wrong kit can lead to incomplete data or artefacts. We assist in selecting the ideal library preparation kit, considering the following:

  • Your sample type (e.g., DNA, RNA).

  • The sequencing platform.

  • The specific goals of your study.

Our guidance helps you avoid issues related to sample degradation or bias in sequencing results.

Evaluating Cost-Effectiveness and Quality

At NextGenSeek, we understand that researchers often face budget constraints. We help you evaluate the cost-effectiveness of different sequencing methods, ensuring you get the highest quality data without overspending. By optimising your experiment design, we help you achieve cost-efficient results that maintain scientific integrity.

Defining the Necessary Controls

Proper controls are essential for reliable NGS experiments. We assist in defining the positive, negative, and technical controls necessary for your specific study, ensuring that your experimental results are robust and reproducible.

Why Pre-Experiment Consultation Matters

Pre-experiment consultation can help you avoid common mistakes derailing NGS projects. Poor planning can lead to unreliable or incomplete data, whether selecting the wrong read length, inadequate coverage depth or failing to account for crucial controls. Our expert guidance ensures your project is strategically designed for success, minimising risks and improving the reliability of your results.

Contact Us Today

At NextGenSeek, we provide tailored consultation services for NGS projects across various research areas, including cancer genomics, microbiome studies, and drug development. Contact us today to discuss your project and learn how our pre-experiment advisory services can help ensure a successful outcome. Our team will work with you to design a comprehensive experimental strategy that meets your research needs and goals.

Let’s ensure your research is set up for success from the start. Contact us here for NGS consultancy or here for general science consultancy.